BIO1
Module-8Metabolism Q1.Explain the First Law of Thermodynamics in Biological Systems?A1. First Law of Thermodynamics in Biological SystemsIn photosynthesis, the main source of energy is supplied by the sun. This Light energy is absorbed by cells present in plant leaves and converted to chemical energy. The chemical energy that is obtained is further stored in the form of glucose, which is later utilised to form complex carbohydrates necessary to build plant mass.The energy that is stored in glucose by plant may be released through a process called cellular respiration. This process allows plant and animal organisms to obtain the energy stored in lipids, carbohydrates, and other macromolecules through the production of ATP. This ATP is necessary and is required to perform cell functions such as DNA replication, Cytosis, mitosis, meiosis, cell movement, exocytosis, and apoptosis.Q2. Define Energy?A2. All biological organisms require energy to survive. In our universe which is a closed system, such as the universe, here the energy is neither consumed but transformed from one form to another. Cells, for example, require energy for a number of processes they perform Living organisms require energy to survive, one such energy is the energy humans get from the food we eat. Human civilization requires energy for all activities, this energy is obtained from sources such as fossil fuels, nuclear fuel, or renewable energy. The processes of Earth's ecosystem and climate and are driven by the radiant energy and the Earth receives natural energy from the sun and the geothermal energy contained within the earth.Energy can be obtained through differentways. Sun is obviously the biggest energy source but there are few chemical reactions organisms can utilize for their benefit too. Autotrophs the primary producers, are organisms that prepare their own food through photosynthesis or chemosynthesis, they use these energy sources to convert sun light or inorganic chemical energy, respectively, to organic chemical energy.Eukaryotes do these in their chloroplasts, prokaryotes do these in many different ways due to the diversity of chemosynthesis. Prokaryotes can convert photosynthetically active radiation (PAR), the part of sunlight that can be used in photosynthesis, to chemical energy with mainly chlorophylls. Same molecules that are in the chloroplasts of eukaryotes.Q3. Define Exothermic and Endothermic reactions?A3. Endothermic ReactionsThe endothermic process, are reactions that take place when the system absorbs energy from its surroundings in the form of heat. Few examples for the endothermic process are ammonium chloride, dry ice, alkanes cracking, photosynthesis, evaporating liquids, melting ice, thermal decomposition, ammonium chloride in water and much more.Exothermic ReactionsThe exothermic reaction is the reverse reaction that occurs in an endothermic reaction. In this process the system releases energy by light or heat to its surrounding. Few examples of this reaction deposition of dry ice, neutralization, burning a substance, respiration, reactions of fuels, deposition of dry ice, solution of sulfuric acid into water
The differences that occur in both reactions, in an Endothermic reaction heat is absorbed into the system, where as in exothermic reactions heat is given out from the system.Q4. What are Energy consuming and Energy yielding reactions?A4. The processes of breaking down carbohydrate molecules or to build these molecules illustrate two types of metabolic pathways. A metabolic pathway is a step-by-step series biochemical reaction that are interconnected and convert a substrate molecule or molecules into a final product or products through a series of metabolic intermediates. For example, one metabolic pathway involves breaking down of carbohydrates into large molecules of glucose. Another metabolic pathway might build glucose and form large carbohydrate molecules used for storage. The first process requires energy and is referred to as anabolic reaction. The second process on the other hand produces energy and is referred to as catabolic reaction. Consequently, metabolism is composed of these two opposite pathways:Anabolism (building molecules) Catabolism (breaking down molecules)
Anabolic and catabolic pathways: Anabolic pathways is a process that require energy to synthesize larger molecules. Catabolic pathways are those that generate energy by breaking down larger molecules. However, both types of pathways are required for maintaining the cell’s energy balance.Anabolic PathwaysAnabolic pathways require an energy input to synthesize complex molecules from simpler ones. A good example of anabolic pathway is the synthesis of sugar from CO2. Other examples include breaking down of amino acid to form large proteins and the synthesis of new DNA strands from nucleic acid building blocks. These processes are very critical to the life of each cell, and take place constantly, and demand energy provided by ATP and other high-energy molecules like NADH (nicotinamide adenine dinucleotide) and NADPH.Catabolic PathwaysCatabolic pathways involve the breaking down of complex molecules into simpler ones, releasing the chemical energy is stored in the bonds of those molecules. Some catabolic pathways can capture the chemical energy to produce ATP, which is the energy molecule used to power all cellular processes. Other energy-storing molecules, such as lipids, are also broken down through similar catabolic reactions to release energy and make ATP. Q5. Define Glycolysis?A5. Glycolysis is the process during which glucose molecules are broken down into two pyruvate molecules, while storing energy released during this process as ATP and NADH. Glycolysis is a process utilised by all organisms. Glucose regulation and product formed are the primary categories in which these pathways differ between organisms. In some tissues and organisms, glycolysis is the only method of energy production. This pathway is common to both anaerobic and aerobic respiration. Glycolysis proceeds in ten steps, split into two phases. During the first phase, two ATP molecules are broken down. During the second phase, chemical energy from the intermediates is transferred into ATP and NADH. The breakdown of one molecule of glucose gives rise to two molecules of pyruvate, which can be further oxidized to access more energy in later processes. Through feedback regulation Glycolysis can be regulated at different steps of the process. The third step is the most regulated one. This regulation is to ensure that the body is not over-producing pyruvate molecules. The regulation also allows for the storage of glucose molecules into fatty acids. There are various enzymes that are used throughout glycolysis. Glycolysis 1. Glycolysis takes place in the cytoplasm 2. It can Occur in the presence or absence of oxygen 3. They Involve ten enzyme-catalysed reactions 4. Broken down into 2 molecules of pyruvate 5. Steps A. Glucose is converted to a 6-C diphosphate. This uses 2 ATP's B. The 6-C molecule is broken down into 2, 3-C molecules C. The 2, 3-C sugars are converted to Pyruvate, gaining 4 ATP and 2 NADH
Q6. Explain Concept of Keq and its relation to Standard free Energy?A6. The free energy change of the reaction in any state, ΔG (when equilibrium has not been attained) is related to the standard free energy change of the reaction, ΔG0 (which is equal to the difference in free energies of formation of the products and reactants both in their standard states) according to the equation. ΔG = ΔGo + RT InQWhere Q is the reaction quotientWhen equilibrium is attained, there is no further free energy change i.e. ΔG = 0 and Q becomes equal to equilibrium constant. Hence the above equation becomes. ΔGo = –RT In K(eq) or ΔGo = –2.303 RT log K(eq)In case of galvanic cells. Gibbs energy change ΔG is related to the electrical work done by the cell.ΔG = -nFE(cell) where n = no. of moles of electrons involved F = the Faraday constant E = emf of the cellIf reactants and products are in their standard states ΔGo = –nFEocellQ7.Explain the Krebs cycle?A7. In the glycolysis cycle pyruvate molecules produced and they contain a lot of energy in the bonds present between their molecules. In order to use that energy, the cell must convert it into the form of ATP, for this to happen pyruvate molecules are processed through the Krebs cycle, also called as the Citric acid cycle. The krebs cycle occurs in the Mitochondria of the cell. Before entering the Krebs cycle, pyruvate has to be converted into acetyl CoA. This is Achieved by the removal of a CO2 molecule from pyruvate and then removing an electro to reduce an NAD+ into NADH. An enzyme called coenzyme A combines with the remaining acetyl to make acetyl CoA, after which it enters the Krebs cycle.The steps in the krebs cycle are summarized below:Citrate is formed when acetyl group from acetyl CoA combines with oxaloacetate Citrate is converted to its isomer, isocitrate Isocitrate is oxidised to form the 5-carbon α-Ketoglutarate. This step releases one molecule of CO2 and reduces NAD+ to NADH2+. The α-Ketoglutarate is oxidised to succinyl CoA yielding CO2andNADH2+ Succinyl CoA releases coenzyme A and Phosphorylates ADP into ATP. Succinate is oxidised to fumarate, converting FAD to FADH2. Fumarate is then hydrolysed to form Malate. Malate is oxidised to oxaloacetate, reducing NAD+ to NADH2+ In the Krebs cycle, during the glycolysis process to pyruvate molecules from one glucose, each glucose is processed through the Krebs cycle two times. For each molecule of glucose ,6 NADH2+,2FADH2 and 2 ATP are formed
Krebs cycle yields (2 cycles,1 for each pyruvate):24 ATP 6 NADH +h ,2 GTP, 2FADH. Q8. What are endergonic and Exergonic Reactions?A8. The word “ender” has derived from the word “endo” meaning from “within”. Therefore, endergonic means these reactions absorb energy in the form of work. therefore, in an endergonic reaction, the surrounding environment supplies energy into the system. Further, the products formed will have a higher energy than the reactants. Endergonic reaction is considered to be nonspontaneous or unfavourable. Gibbs free energy will be positive if this energy transfer takes place in a constant pressure and temperature. Thus, the equilibrium constant for an endergonic reaction is less than one. The best example for this reaction is Photosynthesis which takes places in the natural environment. For photosynthesis, energy is supplied by sunlight. whereas in the human body, when endergonic reactions are taking place, the energy is supplied most of the time by ATP. So, endergonic reactions are coupled to ATP hydrolysis reactions.Exergonic means releasing energy in the form of work. In these reactions, energy is released to the outside from the system. Exergonic reactions are favourable and spontaneous. In this case the products possess less energy than the reactants because the energy is released during the reaction. Therefore, the enthalpy change (∆H) becomes negative. Moreover, if the transfer is carried out in constant pressure and temperature, standard Gibbs free energy will be a negative value.
Q9. Define Electron charge?A9. Electron charge, (symbol e), fundamental physical constant expressing the naturally occurring unit of electric charge, equal to 1.602176634 × 10 −19 coulomb. In addition to the electron, all freely existing charged subatomic particles thus far discovered have an electric charge equal to this value or some whole-number multiple of it.Electrons play an essential role in numerous physical phenomena, such as chemistry, electricity, thermal conductivity and magnetism, and they also participate in electromagnetic, gravitational, and weak interactions. Since an electron has charge, it has a field surrounding it known as the electric field, and if that electron is moving relative to an observer, said observer will observe it to generate a magnetic field. Electrons absorb or radiate energy in the form of photons when they are accelerated. Laboratory instruments are capable of trapping individual electrons as well as electron plasma by the use of electromagnetic fields. Electrons are involved in many other applications. Q10.Write a note on Synthesis of Glucose?A10. Photosynthesis is an important process involving a biochemical pathway that results in the production of sugar (glucose) from sunlight, water and carbon dioxide and in the process releases oxygen. Photosynthesis occurs in few bacteria, algae, higher plants and some photoautotrophs. It occurs in a series of complex biochemical reactions. Nearly all life depends on this process. The rate of photosynthesis is directly related to concentration of carbon dioxide, temperature and light intensity. It gets energy from absorbed photons present on the leaves and uses water as a reducing agent.Carbohydrates are involved in many essential metabolic pathways. Plants utilize sunlight and synthesize carbohydrates from carbon dioxide and water through photosynthesis, energy is stored in the form of carbohydrates through this sunlight internally. In cellular respiration the stored carbohydrate is broken down to release energy by animals and fungi that consume plants. Both animals and plants store the released energy temporarily in the form of high-energy molecules, called ATP, for use in various cellular processes.Photosynthesis is a chemical reaction in which carbon dioxide and water combine to produce glucose and oxygen. Sunlight forms the source of energy, which is absorbed by pigments, called chlorophyll. Chlorophyll is green in colour and is the pigment that gives plants their green colour
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